21-Jan-2023: Researchers have proposed a “Pan-transcriptome” which combines transcriptome with pan-genome

Mapping transcriptome (complete set of RNA molecules) allows researchers to better understand an individual’s gene expression.

Pan-Transcriptome

• It is a Combination of a transcriptome and a pan-genome
• Transcriptome: Complete set of RNA molecules in a cell or tissue, produced by the genetic material of an organism
• Pan-genome: Reference containing genetic material from a diverse group of individuals

Uses

• Discovery of new genes: Can be used to identify novel genes that may not have been detected through traditional genome sequencing methods.
• Regulating gene expression: Pan-transcriptome can change in response to environmental or physiological conditions, and it plays a key role in regulating gene expression.
• Characterizing genetic diversity: Pan-transcriptomes can be used to identify genetic variations within and between different populations of organisms. This can provide insights into the evolution and adaptation of different species.

RNA Mapping

Technique used to identify and map the location of RNA molecules within a cell or tissue. Typically used to study the expression and localization of different types of RNA, including messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA).

Types:

In-situ hybridization (ISH)

• Allows researchers to see where a specific RNA is located within a cell or tissue
• Provides insights into the function of that RNA

RNA-seq (RNA sequencing)

• Simultaneous analysis of thousands or even millions of RNA molecules in a sample
• Can generate a detailed picture of the transcriptome, including the abundance and location of different RNA molecules

Uses

• Study gene expression patterns
• Identify novel transcripts
• Detect genetic variations